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验证数据展示

  • WB analysis of MCF-7 using 29489-1-AP

    WB analysis of MCF-7 using 29489-1-AP

    WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells.

  • WB analysis using 29489-1-AP

    WB analysis using 29489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

PKMYT1 Polyclonal antibody

PKMYT1 Polyclonal Antibody for WB, ELISA
Cat No. 29489-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, A549 cells, MKN-45 cells, NCI-H1299 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29489-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PKMYT1 fusion protein Ag30573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, membrane associated tyrosine/threonine 1
Synonyms DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight60-75 kDa
GenBank Accession NumberNM_004203
Gene Symbol PKMYT1
Gene ID (NCBI) 9088
RRIDAB_3086137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99640
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)

实验方案

Product Specific Protocols
WB protocol for PKMYT1 antibody 29489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols