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验证数据展示

  • WB analysis of MCF-7 using 29489-1-AP

    WB analysis of MCF-7 using 29489-1-AP

    WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells.

  • WB analysis using 29489-1-AP

    WB analysis using 29489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

PKMYT1 Polyclonal antibody

PKMYT1 Polyclonal Antibody for WB, ELISA
Cat No. 29489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, A549 cells, MKN-45 cells, NCI-H1299 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29489-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PKMYT1 fusion protein Ag30573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, membrane associated tyrosine/threonine 1
别名 DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1
计算分子量 55 kDa
观测分子量60-75 kDa
GenBank蛋白编号NM_004203
基因名称 PKMYT1
Gene ID (NCBI) 9088
RRIDAB_3086137
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99640
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)

实验方案

Product Specific Protocols
WB protocol for PKMYT1 antibody 29489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols