PKMYT1 Polyclonal antibody
PKMYT1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A375 cells, HeLa cells, HepG2 cells, HL-60 cells, mouse skin tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
55098-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein kinase, membrane associated tyrosine/threonine 1 |
| Synonyms | MYT1, Myt1 kinase, PKMYT1 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_004203 |
| Gene Symbol | PKMYT1 |
| Gene ID (NCBI) | 9088 |
| RRID | AB_10888636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99640 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PKMYT1 antibody 55098-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thorac Cancer Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3 |
