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Conjugate
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PKNOX1 Polyclonal antibody

PKNOX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10614-1-AP

Print datasheet

Synonyms

PREP1, pkonx1c, Homeobox protein PREP-1

验证数据展示

  • IHC staining of human gliomas using 10614-1-AP

    IHC staining of human gliomas using 10614-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10614-1-AP (PKNOX1 antibody) at dilution of 1:100 (under 25x lens).

  • WB analysis of SH-SY5Y using 10614-1-AP

    WB analysis of SH-SY5Y using 10614-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10614-1-AP targets PKNOX1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PKNOX1 fusion protein Ag0939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PBX/knotted 1 homeobox 1
Synonyms PREP1, pkonx1c, Homeobox protein PREP-1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007746
Gene Symbol PKNOX1
Gene ID (NCBI) 5316
RRIDAB_2164125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKNOX1 antibody 10614-1-APDownload protocol
IHC protocol for PKNOX1 antibody 10614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Am J Hum Genet

Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities.

Authors - Noriko Miyake
View Article
mouseWB,IHC

Am J Physiol Renal Physiol

PMID: 31091119

Authors - Zhiqiang Chen
View Article