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验证数据展示

  • WB analysis using 22632-1-AP

    WB analysis using 22632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22632-1-AP (PKP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 22632-1-AP

    IHC staining of human skin cancer using 22632-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 22632-1-AP (PKP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 22632-1-AP

    IF Staining of PC-3 using 22632-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PKP1 antibody (22632-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Plakophilin 1 Polyclonal antibody

Plakophilin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22632-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PKP1, Plakophilin-1, B6P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHaCaT cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22632-1-AP targets Plakophilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Plakophilin 1 fusion protein Ag18153 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
别名 PKP1, Plakophilin-1, B6P
计算分子量 747 aa, 83 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC114571
基因名称 PKP1
Gene ID (NCBI) 5317
RRIDAB_3085701
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13835
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

实验方案

Product Specific Protocols
WB protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
IHC protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
IF protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols