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Plakophilin 3 Polyclonal antibody

Plakophilin 3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18338-1-AP

Print datasheet

Synonyms

PKP3, Plakophilin-3

验证数据展示

  • WB analysis of K-562 using 18338-1-AP

    WB analysis of K-562 using 18338-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 18338-1-AP

    IP experiment of A549 using 18338-1-AP

    IP result of anti-Plakophilin 3 (IP:18338-1-AP, 4ug; Detection:18338-1-AP 1:600) with A549 cells lysate 1000ug.

  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse skin using 18338-1-AP

    IHC staining of mouse skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 18338-1-AP

    IHC staining of human colon using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human bowen disease using 18338-1-AP

    IHC staining of human bowen disease using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue, human bowen disease, human colon tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18338-1-AP targets Plakophilin 3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen Plakophilin 3 fusion protein Ag13062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plakophilin 3
Synonyms PKP3, Plakophilin-3
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC000081
Gene Symbol Plakophilin 3
Gene ID (NCBI) 11187
RRIDAB_2164281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y446
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IHC protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IP protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

J Ovarian Res

Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis

Authors - Yuan Li
View Article
humanIHC

Biomedicines

Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis

Authors - Yan Du
View Article
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article