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验证数据展示

  • WB analysis of K-562 using 18338-1-AP

    WB analysis of K-562 using 18338-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 18338-1-AP

    IP experiment of A549 using 18338-1-AP

    IP result of anti-Plakophilin 3 (IP:18338-1-AP, 4ug; Detection:18338-1-AP 1:600) with A549 cells lysate 1000ug.

  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse skin using 18338-1-AP

    IHC staining of mouse skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 18338-1-AP

    IHC staining of human colon using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human bowen disease using 18338-1-AP

    IHC staining of human bowen disease using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Plakophilin 3 Polyclonal antibody

Plakophilin 3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18338-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PKP3, Plakophilin-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue, human bowen disease, human colon tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18338-1-AP targets Plakophilin 3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 Plakophilin 3 fusion protein Ag13062 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plakophilin 3
别名 PKP3, Plakophilin-3
计算分子量 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC000081
基因名称 Plakophilin 3
Gene ID (NCBI) 11187
RRIDAB_2164281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y446
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IHC protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IP protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

J Ovarian Res

Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis

Authors - Yuan Li
View Article
humanIHC

Biomedicines

Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis

Authors - Yan Du
View Article
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article