验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, HEK-293 cells, mouse heart tissue, mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
16009-1-AP targets PLA2G12A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PLA2G12A fusion protein Ag8722 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group XIIA |
| 别名 | phospholipase A2, group XIIA, Phosphatidylcholine 2-acylhydrolase 12A, GXII sPLA2, GXII, FKSG38 |
| 计算分子量 | 189 aa, 21 kDa |
| 观测分子量 | 21-24 kDa |
| GenBank蛋白编号 | BC017218 |
| 基因名称 | PLA2G12A |
| Gene ID (NCBI) | 81579 |
| RRID | AB_2164314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZM1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G12A (or PLA2G12) gene encodes group XII secreted phospholipase A2 (sPLA2) which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Cellular arachidonate (AA) release and prostaglandin (PG) production is regulated by sPLA(2)s, groups III and XII. Group XII sPLA2 have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Role of sPLA2s as potential tumour biomarkers and therapeutic targets for various cancers have been reported.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLA2G12A antibody 16009-1-AP | Download protocol |
| IHC protocol for PLA2G12A antibody 16009-1-AP | Download protocol |
| IF protocol for PLA2G12A antibody 16009-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cardiol Phospholipase A2 expression in coronary thrombus is increased in patients with recurrent cardiac events after acute myocardial infarction. | ||
Atherosclerosis Quantitative trait locus mapping in mice identifies phospholipase Pla2g12a as novel atherosclerosis modifier. | ||
Exp Ther Med Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease. | ||
J Atheroscler Thromb The Expression of Groups IIE and V Phospholipase A2 is Associated with an Increased Expression of Osteogenic Molecules in Human Calcified Aortic Valves. | ||
Nat Commun Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis |
