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LYPLA3 Polyclonal antibody

LYPLA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10863-2-AP

Print datasheet

Synonyms

1 O acylceramide synthase, ACS, Group XV phospholipase A2, GXVPLA2, LCAT like lysophospholipase, LLPL, LPLA2, LYPLA3, Lysophospholipase 3, Lysosomal phospholipase A2, phospholipase A2, group XV, PLA2G15

验证数据展示

  • IHC staining of human liver cancer using 10863-2-AP

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 40x lens)..

  • IHC staining of human liver cancer using 10863-2-AP

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 10x lens)..

  • WB analysis of mouse spleen using 10863-2-AP

    WB analysis of mouse spleen using 10863-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10863-2-AP (LYPLA3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse spleen tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

10863-2-AP targets LYPLA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen LYPLA3 fusion protein Ag1304 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group XV
Synonyms 1 O acylceramide synthase, ACS, Group XV phospholipase A2, GXVPLA2, LCAT like lysophospholipase, LLPL, LPLA2, LYPLA3, Lysophospholipase 3, Lysosomal phospholipase A2, phospholipase A2, group XV, PLA2G15
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 42-57 kDa
GenBank Accession NumberBC011640
Gene Symbol PLA2G15
Gene ID (NCBI) 23659
RRIDAB_2283832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G15 is also named as LYPLA3 and belongs to the AB hydrolase superfamily. It has transacylase and calcium-independent phospholipase A2 activity. PLA2G15 catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. It can be detected as 42-47 kDa and 57 kDa due to the N-glycosylated modification (PMID:11790796).

实验方案

Product Specific Protocols
WB protocol for LYPLA3 antibody 10863-2-APDownload protocol
IHC protocol for LYPLA3 antibody 10863-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

bioRxiv

Loss of the lysosomal protein CLN3 modifies the lipid content of the nuclear envelope leading to DNA damage and activation of YAP1 pro-apoptotic signaling

Authors - Neuza Domingues
View Article