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验证数据展示

  • WB analysis using 15843-1-AP

    WB analysis using 15843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15843-1-AP

    WB analysis using 15843-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 15843-1-AP

    IHC staining of human pancreas cancer using 15843-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15843-1-AP

    IHC staining of human pancreas cancer using 15843-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 15843-1-AP

    IF Staining of mouse pancreas using 15843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLA2G1B Polyclonal antibody

PLA2G1B Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 15843-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue, mouse spleen tissue, rat pancreas tissue, pig pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15843-1-AP targets PLA2G1B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 PLA2G1B fusion protein Ag8603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IB (pancreas)
别名 PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4
计算分子量70aa,8 kDa; 148aa,16 kDa
观测分子量 13-16 kDa
GenBank蛋白编号BC005386
基因名称 PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2878190
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04054
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

实验方案

Product Specific Protocols
WB protocol for PLA2G1B antibody 15843-1-APDownload protocol
IHC protocol for PLA2G1B antibody 15843-1-APDownload protocol
IF protocol for PLA2G1B antibody 15843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Food Chem

Dietary sn-2 palmitate influences cognitive behavior by increasing the transport of liver-produced lysophosphatidylcholine VLCPUFAs to the brain

Authors - Teng Wei
View Article