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验证数据展示

  • WB analysis of pig pancreas using 66397-1-Ig

    WB analysis of pig pancreas using 66397-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens).

PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66397-1-Ig

产品说明书

CloneNo. 1F2C10

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 PLA2G1B fusion protein Ag8812 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2, group IB (pancreas)
别名 Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2
计算分子量70aa,8 kDa; 148aa,16 kDa
观测分子量 13-16 kDa
GenBank蛋白编号BC005386
基因名称 PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04054
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

实验方案

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols