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PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C10

Cat No : 66397-1-Ig

Print datasheet

Synonyms

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

验证数据展示

  • WB analysis of pig pancreas using 66397-1-Ig

    WB analysis of pig pancreas using 66397-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, pig
Immunogen PLA2G1B fusion protein Ag8812 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IB (pancreas)
Synonyms Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2
Calculated Molecular Weight70aa,8 kDa; 148aa,16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC005386
Gene Symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04054
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

实验方案

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols