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验证数据展示

  • WB analysis using 21004-1-AP

    WB analysis using 21004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21004-1-AP (PLA2G4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PLA2G4A Polyclonal antibody

PLA2G4A Polyclonal Antibody for WB, ELISA
Cat No. 21004-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, cPLA2 alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MG-63 cells, NIH/3T3 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21004-1-AP targets PLA2G4A in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PLA2G4A fusion protein Ag15202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, cPLA2 alpha
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).

实验方案

Product Specific Protocols
WB protocol for PLA2G4A antibody 21004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols