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PLA2G4A Polyclonal antibody

PLA2G4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28924-1-AP

Print datasheet

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

验证数据展示

  • WB analysis using 28924-1-AP

    WB analysis using 28924-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28924-1-AP (PLA2G4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28924-1-AP

    IHC staining of human colon cancer using 28924-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28924-1-AP (PLA2G4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28924-1-AP

    IHC staining of human colon cancer using 28924-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28924-1-AP (PLA2G4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28924-1-AP targets PLA2G4A in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).

实验方案

Product Specific Protocols
WB protocol for PLA2G4A antibody 28924-1-APDownload protocol
IHC protocol for PLA2G4A antibody 28924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols