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  • Featured Product
  • KD/KO Validated

PLAA Polyclonal antibody

PLAA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12529-1-AP

Print datasheet

Synonyms

DOA1, PLA2P, PLAA, PLAP

验证数据展示

  • WB analysis of MCF-7 using 12529-1-AP

    WB analysis of MCF-7 using 12529-1-AP

    WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • WB analysis of K-562 using 12529-1-AP

    WB analysis of K-562 using 12529-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12529-1-AP

    IHC staining of human lung cancer using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLAA antibody included
  • IHC staining of human lung cancer using 12529-1-AP

    IHC staining of human lung cancer using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLAA antibody included
  • IP experiment of K-562 using 12529-1-AP

    IP experiment of K-562 using 12529-1-AP

    IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLAA Ready-To-Use IHC Kit. PLAA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12529-1-AP targets PLAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PLAA fusion protein Ag3224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2-activating protein
Synonyms DOA1, PLA2P, PLAA, PLAP
Calculated Molecular Weight 738 aa, 81 kDa
Observed Molecular Weight 72-74 kDa
GenBank Accession NumberBC032551
Gene Symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2164881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

实验方案

Product Specific Protocols
WB protocol for PLAA antibody 12529-1-APDownload protocol
IHC protocol for PLAA antibody 12529-1-APDownload protocol
IP protocol for PLAA antibody 12529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IHC

Anesthesiology

miR-203 regulates nociceptive sensitization after incision by controlling phospholipase A2 activating protein expression.

Authors - Sun Yuan Y
View Article
humanWB,IF

Biochim Biophys Acta Mol Cell Res

Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization

Authors - Zhilong Zheng
  • KD Validated
View Article