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验证数据展示

  • WB analysis of MCF-7 using 60244-1-Ig

    WB analysis of MCF-7 using 60244-1-Ig

    WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • WB analysis of ROS1728 using 60244-1-Ig

    WB analysis of ROS1728 using 60244-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PLAA antibody included
  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PLAA antibody included
  • Featured Product
  • KD/KO验证

PLAA Monoclonal antibody

PLAA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60244-1-Ig

产品说明书

CloneNo. 5C10G9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DOA1, PLA2P, PLAA, PLAP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells, K-562 cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLAA Ready-To-Use IHC Kit. PLAA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60244-1-Ig targets PLAA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PLAA fusion protein Ag3224 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2-activating protein
别名 DOA1, PLA2P, PLAA, PLAP
计算分子量 738 aa, 81 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC032551
基因名称 PLAA
Gene ID (NCBI) 9373
RRIDAB_2881366
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

实验方案

Product Specific Protocols
WB protocol for PLAA antibody 60244-1-IgDownload protocol
IHC protocol for PLAA antibody 60244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols