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PLAGL1 Polyclonal antibody

PLAGL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25864-1-AP

Print datasheet

Synonyms

Tumor suppressor ZAC, Pleiomorphic adenoma-like protein 1, LOT-1, LOT1, LOT 1

验证数据展示

  • WB analysis of MCF-7 using 25864-1-AP

    WB analysis of MCF-7 using 25864-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25864-1-AP (PLAGL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 25864-1-AP

    IHC staining of human stomach cancer using 25864-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25864-1-AP (PLAGL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, mouse heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25864-1-AP targets PLAGL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PLAGL1 fusion protein Ag23073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleiomorphic adenoma gene-like 1
Synonyms Tumor suppressor ZAC, Pleiomorphic adenoma-like protein 1, LOT-1, LOT1, LOT 1
Calculated Molecular Weight 463 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC074814
Gene Symbol PLAGL1
Gene ID (NCBI) 5325
RRIDAB_2880274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLAGL1 antibody 25864-1-APDownload protocol
IHC protocol for PLAGL1 antibody 25864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols