- Featured Product
- KD/KO Validated
t-Plasminogen activator/tPA Polyclonal antibody
t-Plasminogen activator/tPA Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, mouse pancreas tissue, rat kidney tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10147-1-AP targets t-Plasminogen activator/tPA in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | t-Plasminogen activator/tPA fusion protein Ag0200 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | plasminogen activator, tissue |
Synonyms | PLAT, tPA, T PA, Reteplase, EC:3.4.21.68 |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 32-35 kDa, 65 kDa |
GenBank Accession Number | BC002795 |
Gene Symbol | tPA |
Gene ID (NCBI) | 5327 |
RRID | AB_2299701 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00750 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for t-Plasminogen activator/tPA antibody 10147-1-AP | Download protocol |
IHC protocol for t-Plasminogen activator/tPA antibody 10147-1-AP | Download protocol |
IF protocol for t-Plasminogen activator/tPA antibody 10147-1-AP | Download protocol |
IP protocol for t-Plasminogen activator/tPA antibody 10147-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.
| ||
J Neurosci Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice. | ||
Aging (Albany NY) NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium. | ||
Glia Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures. | ||
Sci Rep Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells. | ||
Cell Cycle tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis. |