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PLCB2 Polyclonal antibody

PLCB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27456-1-AP

Print datasheet

Synonyms

PLC beta 2, Phospholipase C-beta-2, Phospholipase C beta 2, Phosphoinositide phospholipase C-beta-2, EC:3.1.4.11

验证数据展示

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27456-1-AP

    IHC staining of mouse liver using 27456-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27456-1-AP (PLCB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 27456-1-AP

    IF Staining of THP-1 using 27456-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PLCB2 antibody (27456-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27456-1-AP targets PLCB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PLCB2 fusion protein Ag25771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, beta 2
Synonyms PLC beta 2, Phospholipase C-beta-2, Phospholipase C beta 2, Phosphoinositide phospholipase C-beta-2, EC:3.1.4.11
Calculated Molecular Weight 134 kDa
Observed Molecular Weight 134 kDa
GenBank Accession NumberBC000939
Gene Symbol PLCB2
Gene ID (NCBI) 5330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCB2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2) is also named as PLC-beta-2. PLCB2 is a critical regulator of platelet responses upon activation (PMID: 27465150). NF-κB regulates expression of PLC-β2. The PLCB2 genes was found to be differentially expressed in human breast cancer MCF-7 cells, and to be associated with multidrug resistance (PMID: 2951275). The knockdown of PLCB2 suppressed cell viability and promoted cell apoptosis by activating the Ras/Raf/MAPK pathway. Thus, PLCB2 may utilized as a potential therapeutic target in patients with melanoma (PMID: 31746389).

实验方案

Product Specific Protocols
WB protocol for PLCB2 antibody 27456-1-APDownload protocol
IHC protocol for PLCB2 antibody 27456-1-APDownload protocol
IF protocol for PLCB2 antibody 27456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols