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验证数据展示

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27456-1-AP

    IHC staining of mouse liver using 27456-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27456-1-AP (PLCB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27456-1-AP

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 27456-1-AP

    IF Staining of THP-1 using 27456-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PLCB2 antibody (27456-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PLCB2 Polyclonal antibody

PLCB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27456-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PLC beta 2, Phospholipase C-beta-2, Phospholipase C beta 2, Phosphoinositide phospholipase C-beta-2, EC:3.1.4.11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27456-1-AP targets PLCB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PLCB2 fusion protein Ag25771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, beta 2
Synonyms PLC beta 2, Phospholipase C-beta-2, Phospholipase C beta 2, Phosphoinositide phospholipase C-beta-2, EC:3.1.4.11
Calculated Molecular Weight 134 kDa
Observed Molecular Weight 134 kDa
GenBank Accession NumberBC000939
Gene Symbol PLCB2
Gene ID (NCBI) 5330
RRIDAB_3085963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCB2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2) is also named as PLC-beta-2. PLCB2 is a critical regulator of platelet responses upon activation (PMID: 27465150). NF-κB regulates expression of PLC-β2. The PLCB2 genes was found to be differentially expressed in human breast cancer MCF-7 cells, and to be associated with multidrug resistance (PMID: 2951275). The knockdown of PLCB2 suppressed cell viability and promoted cell apoptosis by activating the Ras/Raf/MAPK pathway. Thus, PLCB2 may utilized as a potential therapeutic target in patients with melanoma (PMID: 31746389).

实验方案

Product Specific Protocols
WB protocol for PLCB2 antibody 27456-1-APDownload protocol
IHC protocol for PLCB2 antibody 27456-1-APDownload protocol
IF protocol for PLCB2 antibody 27456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols