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PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21370-1-AP

Print datasheet

Synonyms

Phospholipase C beta 3, PLC beta 3, PLCB3

验证数据展示

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of MCF-7 using 21370-1-AP

    WB analysis of MCF-7 using 21370-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293T cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21370-1-AP targets PLCB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PLCB3 fusion protein Ag15845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Synonyms Phospholipase C beta 3, PLC beta 3, PLCB3
Calculated Molecular Weight 1234 aa, 139 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2878848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCB3 antibody 21370-1-APDownload protocol
IHC protocol for PLCB3 antibody 21370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols