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PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21370-1-AP

Print datasheet

Synonyms

Phospholipase C beta 3, PLC beta 3, PLCB3

验证数据展示

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of MCF-7 using 21370-1-AP

    WB analysis of MCF-7 using 21370-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293T cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21370-1-AP is PLCB3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PLCB3 fusion protein Ag15845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Synonyms Phospholipase C beta 3, PLC beta 3, PLCB3
Calculated Molecular Weight 1234 aa, 139 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2878848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCB3 antibody 21370-1-APDownload protocol
IHC protocol for PLCB3 antibody 21370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols