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PLCB3 Monoclonal antibody, PBS Only

PLCB3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8B3

Cat no : 66668-1-PBS

Print datasheet

Synonyms

Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

验证数据展示

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

产品信息

66668-1-PBS targets PLCB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PLCB3 fusion protein Ag15845 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Synonyms Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
Calculated Molecular Weight 1234 aa, 139 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01970
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.