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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

PLCB3 Monoclonal antibody, PBS Only

PLCB3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66668-1-PBS

产品说明书

CloneNo. 1B8B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66668-1-PBS targets PLCB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PLCB3 fusion protein Ag15845 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase C, beta 3 (phosphatidylinositol-specific)
别名 Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
计算分子量 1234 aa, 139 kDa
观测分子量 150 kDa
GenBank蛋白编号BC142681
基因名称 PLCB3
Gene ID (NCBI) 5331
RRIDAB_2882022
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01970
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.