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PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10589-2-AP

Print datasheet

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

验证数据展示

  • WB analysis of mouse skeletal muscle using 10589-2-AP

    WB analysis of mouse skeletal muscle using 10589-2-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10589-2-AP

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10589-2-AP

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10589-2-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PLCD4 fusion protein Ag0937 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, delta 4
Synonyms hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4
Calculated Molecular Weight 85-90 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
RRIDAB_2163398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

实验方案

Product Specific Protocols
WB protocol for PLCD4 antibody 10589-2-APDownload protocol
IHC protocol for PLCD4 antibody 10589-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols