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PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30133-1-AP

Print datasheet

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

验证数据展示

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells.

  • IHC staining of mouse testis using 30133-1-AP

    IHC staining of mouse testis using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 30133-1-AP

    IHC staining of human pancreas cancer using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, rat testis tissue, human testis tissue
Positive IHC detected inmouse testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30133-1-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen PLCD4 fusion protein Ag32651 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, delta 4
Synonyms hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4
Calculated Molecular Weight 85-90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).

实验方案

Product Specific Protocols
WB protocol for PLCD4 antibody 30133-1-APDownload protocol
IHC protocol for PLCD4 antibody 30133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols