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验证数据展示

  • IF Staining of Jurkat using 84941-1-RR

    IF Staining of Jurkat using 84941-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HEK-293 using 84941-1-RR

    IF Staining of HEK-293 using 84941-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PLCG1 Recombinant antibody

PLCG1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 84941-1-RR

产品说明书

CloneNo. 242529A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 242529A11, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-1, Phospholipase C gamma 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84941-1-RR targets PLCG1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PLCG1 fusion protein Ag27218 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phospholipase C, gamma 1
别名 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 242529A11, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-1, Phospholipase C gamma 1
计算分子量 149 kDa
基因名称 PLCG1
Gene ID (NCBI) 5335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19174
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.

实验方案

Product Specific Protocols
IF protocol for PLCG1 antibody 84941-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols