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验证数据展示

  • WB analysis using 27173-1-AP

    WB analysis using 27173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27173-1-AP (PLCG2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27173-1-AP

    IHC staining of human tonsillitis using 27173-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCG2 antibody included
  • IHC staining of human tonsillitis using 27173-1-AP

    IHC staining of human tonsillitis using 27173-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCG2 antibody included

PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27173-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Phospholipase C gamma 2, Phospholipase C IV, PLC gamma 2, PLC IV, PLC γ2, PLCG2, PLCγ2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27173-1-AP targets PLCG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PLCG2 fusion protein Ag25847 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, gamma 2 (phosphatidylinositol-specific)
别名 Phospholipase C gamma 2, Phospholipase C IV, PLC gamma 2, PLC IV, PLC γ2, PLCG2, PLCγ2
计算分子量 148 kDa
观测分子量 148 kDa
GenBank蛋白编号BC007565
基因名称 PLCG2
Gene ID (NCBI) 5336
RRIDAB_2880785
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16885
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCG2 antibody 27173-1-APDownload protocol
IHC protocol for PLCG2 antibody 27173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols