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PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29567-1-AP

Print datasheet

Synonyms

Phospholipase C-gamma-2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11

验证数据展示

  • WB analysis using 29567-1-AP

    WB analysis using 29567-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29567-1-AP (PLCG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-937 using 29567-1-AP

    IF Staining of U-937 using 29567-1-AP

    Immunofluorescent analysis of un-fixed Live U-937 cells using PLCG2 antibody (29567-1-AP) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IF/ICC detected inLive U-937 cells
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29567-1-AP targets PLCG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Synonyms Phospholipase C-gamma-2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11
Calculated Molecular Weight 148 kDa
Observed Molecular Weight148 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
RRIDAB_2918326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

实验方案

Product Specific Protocols
WB protocol for PLCG2 antibody 29567-1-APDownload protocol
IF protocol for PLCG2 antibody 29567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols