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验证数据展示

  • WB analysis using 29567-1-AP

    WB analysis using 29567-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29567-1-AP (PLCG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-937 using 29567-1-AP

    IF Staining of U-937 using 29567-1-AP

    Immunofluorescent analysis of un-fixed Live U-937 cells using PLCG2 antibody (29567-1-AP) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29567-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Phospholipase C-gamma-2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IF/ICC detected inLive U-937 cells
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29567-1-AP targets PLCG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, gamma 2 (phosphatidylinositol-specific)
别名 Phospholipase C-gamma-2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11
计算分子量 148 kDa
观测分子量148 kDa
GenBank蛋白编号BC007565
基因名称 PLCG2
Gene ID (NCBI) 5336
RRIDAB_2918326
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16885
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

实验方案

Product Specific Protocols
WB protocol for PLCG2 antibody 29567-1-APDownload protocol
IF protocol for PLCG2 antibody 29567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols