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PLCH1 Polyclonal antibody

PLCH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19143-1-AP

Print datasheet

Synonyms

Phospholipase C-eta-1, phospholipase C, eta 1, Phospholipase C like protein 3, Phosphoinositide phospholipase C-eta-1, EC:3.1.4.11

验证数据展示

  • WB analysis using 19143-1-AP

    WB analysis using 19143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19143-1-AP (PLCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19143-1-AP

    IHC staining of human brain using 19143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 19143-1-AP

    IHC staining of human brain using 19143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19143-1-AP

    IF Staining of HeLa using 19143-1-AP

    Immunofluorescent analysis of Hela cells, using PLCH1 antibody 19143-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19143-1-AP targets PLCH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PLCH1 fusion protein Ag13607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, eta 1
Synonyms Phospholipase C-eta-1, phospholipase C, eta 1, Phospholipase C like protein 3, Phosphoinositide phospholipase C-eta-1, EC:3.1.4.11
Calculated Molecular Weight 1655 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC113950
Gene Symbol PLCH1
Gene ID (NCBI) 23007
RRIDAB_10643386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4KWH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCH1 antibody 19143-1-APDownload protocol
IHC protocol for PLCH1 antibody 19143-1-APDownload protocol
IF protocol for PLCH1 antibody 19143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics

Authors - Wei Liu
View Article