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验证数据展示

  • WB analysis using 17421-1-AP

    WB analysis using 17421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17421-1-AP (PLCL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 17421-1-AP

    IF Staining of mouse brain using 17421-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 17421-1-AP (PLCL2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

PLCL2 Polyclonal antibody

PLCL2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 17421-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLC ε2, PLCE2, PLCL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17421-1-AP targets PLCL2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 PLCL2 fusion protein Ag11443 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C-like 2
别名 KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLC ε2, PLCE2, PLCL2
计算分子量 126 kDa
观测分子量 126 kDa
GenBank蛋白编号BC036392
基因名称 PLCL2
Gene ID (NCBI) 23228
RRIDAB_2163732
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 17421-1-AP antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

实验方案

Product Specific Protocols
WB protocol for PLCL2 antibody 17421-1-APDownload protocol
IF protocol for PLCL2 antibody 17421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility.

Authors - Mary C Peavey
View Article
mouseWB

Front Physiol

Release of GLP-1 and PYY in response to the activation of G protein-coupled bile acid receptor TGR5 is mediated by Epac/PLC-ε pathway and modulated by endogenous H2S.

Authors - Vanitha Bala
View Article