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验证数据展示

  • WB analysis using 17327-1-AP

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 17327-1-AP

    IHC staining of human stomach using 17327-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 17327-1-AP

    IF Staining of mouse brain using 17327-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 17327-1-AP

    IF Staining of A431 using 17327-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PLD3 Polyclonal antibody

PLD3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 17327-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

Hu-K4, HU K4, HindIII K4L homolog, EC:3.1.16.1, Choline phosphatase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17327-1-AP targets PLD3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PLD3 fusion protein Ag11306 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase D family, member 3
别名 Hu-K4, HU K4, HindIII K4L homolog, EC:3.1.16.1, Choline phosphatase 3
计算分子量 490 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC036327
基因名称 PLD3
Gene ID (NCBI) 23646
RRIDAB_2878383
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IV08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLD3 antibody 17327-1-APDownload protocol
IHC protocol for PLD3 antibody 17327-1-APDownload protocol
IF protocol for PLD3 antibody 17327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.

Authors - Teresa R Kee
View Article
humanFC

Cell Death Discov

A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.

Authors - Amy C Flor
View Article