验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10891-2-AP targets PLDN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PLDN fusion protein Ag1337 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pallidin homolog (mouse) |
| 别名 | BLOC1S6, PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN |
| 计算分子量 | 23~24 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC004819 |
| 基因名称 | PLDN |
| Gene ID (NCBI) | 26258 |
| RRID | AB_2164174 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL45 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion. Pallidin is a 25 kDa subunit of the octamer BLOC-1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLDN antibody 10891-2-AP | Download protocol |
| IHC protocol for PLDN antibody 10891-2-AP | Download protocol |
| IP protocol for PLDN antibody 10891-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol BLOC-1 Brings Together the Actin and Microtubule Cytoskeletons to Generate Recycling Endosomes.
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Am J Respir Cell Mol Biol Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.
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