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验证数据展示

  • WB analysis using 29170-1-AP

    WB analysis using 29170-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29170-1-AP

    IHC staining of mouse heart using 29170-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLEC antibody included
  • IF Staining of A549 using 29170-1-AP

    IF Staining of A549 using 29170-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PLEC Polyclonal antibody

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29170-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PLEC Ready-To-Use IHC Kit. PLEC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29170-1-AP targets PLEC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PLEC fusion protein Ag29418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plectin 1, intermediate filament binding protein 500kDa
别名 PLEC1, Plectin, PLTN, Plectin 1, PLEC1b
计算分子量 532 kDa
观测分子量 510 kDa
GenBank蛋白编号NM_201380
基因名称 Plectin
Gene ID (NCBI) 5339
RRIDAB_3086100
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15149
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

实验方案

Product Specific Protocols
WB protocol for PLEC antibody 29170-1-APDownload protocol
IHC protocol for PLEC antibody 29170-1-APDownload protocol
IF protocol for PLEC antibody 29170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols