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PLEC Polyclonal antibody

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29170-1-AP

Print datasheet

Synonyms

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

验证数据展示

  • WB analysis using 29170-1-AP

    WB analysis using 29170-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29170-1-AP

    IHC staining of mouse heart using 29170-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 29170-1-AP

    IF Staining of A549 using 29170-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29170-1-AP targets PLEC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PLEC fusion protein Ag29418 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plectin 1, intermediate filament binding protein 500kDa
Synonyms PLEC1, Plectin, PLTN, Plectin 1, PLEC1b
Calculated Molecular Weight 532 kDa
Observed Molecular Weight 510 kDa
GenBank Accession NumberNM_201380
Gene Symbol Plectin
Gene ID (NCBI) 5339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15149
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

实验方案

Product Specific Protocols
WB protocol for PLEC antibody 29170-1-APDownload protocol
IHC protocol for PLEC antibody 29170-1-APDownload protocol
IF protocol for PLEC antibody 29170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols