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验证数据展示

  • WB analysis of HeLa using 26830-1-AP

    WB analysis of HeLa using 26830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 26830-1-AP

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 26830-1-AP

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 26830-1-AP

    WB analysis using 26830-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 26830-1-AP

    IP experiment of A549 using 26830-1-AP

    IP result of anti-PLEKHA1 (IP:26830-1-AP, 4ug; Detection:26830-1-AP 1:1000) with A549 cells lysate 1600 ug.

  • IF Staining of A549 using 26830-1-AP

    IF Staining of A549 using 26830-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEKHA1 antibody (26830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26830-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26830-1-AP targets PLEKHA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 PLEKHA1 fusion protein Ag25285 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
别名 TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC001136
基因名称 PLEKHA1
Gene ID (NCBI) 59338
RRIDAB_2880651
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

实验方案

Product Specific Protocols
WB protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IHC protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IF protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IP protocol for PLEKHA1 antibody 26830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols