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PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26830-1-AP

Print datasheet

Synonyms

TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1

验证数据展示

  • WB analysis of HeLa using 26830-1-AP

    WB analysis of HeLa using 26830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 26830-1-AP

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 26830-1-AP

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 26830-1-AP

    WB analysis using 26830-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 26830-1-AP

    IP experiment of A549 using 26830-1-AP

    IP result of anti-PLEKHA1 (IP:26830-1-AP, 4ug; Detection:26830-1-AP 1:1000) with A549 cells lysate 1600 ug.

  • IF Staining of A549 using 26830-1-AP

    IF Staining of A549 using 26830-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEKHA1 antibody (26830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26830-1-AP targets PLEKHA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen PLEKHA1 fusion protein Ag25285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC001136
Gene Symbol PLEKHA1
Gene ID (NCBI) 59338
RRIDAB_2880651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

实验方案

Product Specific Protocols
WB protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IHC protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IF protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IP protocol for PLEKHA1 antibody 26830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols