验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10512-1-AP targets PLEKHB1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PLEKHB1 fusion protein Ag0797 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family B (evectins) member 1 |
| 别名 | Evectin 1, EVT1, KPL1, PHR1, PHRET1, PLEKHB1 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC008075 |
| 基因名称 | PLEKHB1 |
| Gene ID (NCBI) | 58473 |
| RRID | AB_2166943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UF11 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHB1, also named as Evectin-1, EVT1, KPL1, PHR1 and PHRET1, is required for proper localization of retinogeniculate projections but not for eye-specific segregation. PLEKHB1 has 4 isoforms with MW from 21-27kd. It has Dimer form with MW 42-54 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHB1 antibody 10512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |