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PLEKHB2 Monoclonal antibody

PLEKHB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H9D7

Cat no : 67877-1-Ig

Print datasheet

Synonyms

Evectin 2, EVT2, FLJ20783, FLJ23679, FLJ30436, PLEKHB2

验证数据展示

  • WB analysis using 67877-1-Ig

    WB analysis using 67877-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67877-1-Ig (PLEKHB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inCaco-2 cells, HT-29 cells, COLO320 cells, SW480 cells, NCCIT cells, BxPC-3 cells, U-87 MG cells, HL-60 cells, Pig colon tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67877-1-Ig targets PLEKHB2 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen PLEKHB2 fusion protein Ag9231 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pleckstrin homology domain containing, family B (evectins) member 2
Synonyms Evectin 2, EVT2, FLJ20783, FLJ23679, FLJ30436, PLEKHB2
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight19-25;30-34 kDa
GenBank Accession NumberBC013991
Gene Symbol PLEKHB2
Gene ID (NCBI) 55041
RRIDAB_2918634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLEKHB2, also named as EVT2 (Evectin-2), is involved in retrograde transport of recycling endosomes. PLEKHB2 has been identified as post-Golgi proteins of unknown function that have a PH domain that typically binds polyphosphoinositides. PLEKHB2 is expressed in a broad range of tissues. PLEKHB2 has some isoforms with MW 19-26 kDa and 34 kDa. This antibody recognizes all the isoforms of PLEKHB2.

实验方案

Product Specific Protocols
WB protocol for PLEKHB2 antibody 67877-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols