验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, human kidney tissue, mouse lung tissue, mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20389-1-AP targets PLEKHF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PLEKHF1 fusion protein Ag14228 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family F (with FYVE domain) member 1 |
| 别名 | Apoptosis inducing protein, APPD, LAPF, Phafin 1, PHAFIN1, PLEKHF1, ZFYVE15 |
| 计算分子量 | 279 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC002744 |
| 基因名称 | PLEKHF1 |
| Gene ID (NCBI) | 79156 |
| RRID | AB_10666430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96S99 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHF1 antibody 20389-1-AP | Download protocol |
| IHC protocol for PLEKHF1 antibody 20389-1-AP | Download protocol |
| IF protocol for PLEKHF1 antibody 20389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Breast Cancer Res Functional characterization of the 19q12 amplicon in grade III breast cancers.
| ||
Sci China Life Sci Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization
|
