验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
14204-1-AP targets PLEKHH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PLEKHH2 fusion protein Ag5409 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 |
| 别名 | KIAA2028, PLEKHH1L, PLEKHH2 |
| 计算分子量 | 168 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC063310 |
| 基因名称 | PLEKHH2 |
| Gene ID (NCBI) | 130271 |
| RRID | AB_2878027 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVE3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHH2 is a 1491-residue intracellular protein highly enriched in renal glomerular podocytes. PLEKHH2 is one of two previously uncharacterized PLEKHH proteins encoded in the mammalian genomes. A PLEKHH ortholog has also been described in Drosophila, Caenorhabditis elegans, and zebrafish. In C. elegans, the loss of the protein was found to cause variable axon guidance defects.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHH2 antibody 14204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |