验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28154-1-AP targets PLEKHH3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 | PLEKHH3 fusion protein Ag28001 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family H (with MyTH4 domain) member 3 |
| 别名 | FLJ21019, PLEKHH3 |
| 计算分子量 | 793 aa, 85 kDa |
| 观测分子量 | 85 and 80 kDa |
| GenBank蛋白编号 | BC052978 |
| 基因名称 | PLEKHH3 |
| Gene ID (NCBI) | 79990 |
| RRID | AB_2881076 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHH3, a gene related to endothelial tight junction, is associated with obstructive sleep apnea (OSA) and angiogenesis. It has been reported that the expression of PLEKHH3 protein was increased in the OSA patients. PLEKHH3 may play a key role in OSA and angiogenesis by specifically regulating the endothelial cells formation of tight junctions. 28154-1-AP detects the isoforms of PLEKHH3 protein around 80-85 kDa in SDS-PAGE.(PMID:28520763)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHH3 antibody 28154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
