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PLEKHO2 Polyclonal antibody

PLEKHO2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21356-1-AP

Print datasheet

Synonyms

PLEKHO2, PLEKHQ1, PP1628, pp9099

验证数据展示

  • IHC staining of mouse spleen using 21356-1-AP

    IHC staining of mouse spleen using 21356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 21356-1-AP

    WB analysis of HeLa using 21356-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of A431 using 21356-1-AP

    IF Staining of A431 using 21356-1-AP

    Immunofluorescent analysis of A431 cells using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 21356-1-AP is PLEKHO2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PLEKHO2 fusion protein Ag15786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain containing, family O member 2
Synonyms PLEKHO2, PLEKHQ1, PP1628, pp9099
Calculated Molecular Weight 490 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC008744
Gene Symbol PLEKHO2
Gene ID (NCBI) 80301
RRIDAB_2878846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD55
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)'s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).

实验方案

Product Specific Protocols
WB protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IHC protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IF protocol for PLEKHO2 antibody 21356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Exploring the immune microenvironment of osteosarcoma through T cell exhaustion-associated gene expression: a study on prognosis prediction

Authors - Junchao Zhu
View Article