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验证数据展示

  • IHC staining of mouse spleen using 21356-1-AP

    IHC staining of mouse spleen using 21356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 21356-1-AP

    WB analysis of HeLa using 21356-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of A431 using 21356-1-AP

    IF Staining of A431 using 21356-1-AP

    Immunofluorescent analysis of A431 cells using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PLEKHO2 Polyclonal antibody

PLEKHO2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21356-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

PLEKHO2, PLEKHQ1, PP1628, pp9099

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21356-1-AP targets PLEKHO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 PLEKHO2 fusion protein Ag15786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology domain containing, family O member 2
别名 PLEKHO2, PLEKHQ1, PP1628, pp9099
计算分子量 490 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC008744
基因名称 PLEKHO2
Gene ID (NCBI) 80301
RRIDAB_2878846
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD55
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)'s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).

实验方案

Product Specific Protocols
WB protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IHC protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IF protocol for PLEKHO2 antibody 21356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Exploring the immune microenvironment of osteosarcoma through T cell exhaustion-associated gene expression: a study on prognosis prediction

Authors - Junchao Zhu
View Article