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Perilipin 1 Recombinant antibody

Perilipin 1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240994H1

Cat no : 83905-4-RR

Print datasheet

Synonyms

PLIN, PLIN1, PERI, 240994H1

验证数据展示

  • WB analysis using 83905-4-RR

    WB analysis using 83905-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83905-4-RR

    Affinity and Kinetic Characterization of 83905-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells
Planning an IHC experiment? We recommend our IHCeasy PLIN1 Ready-To-Use IHC Kit. PLIN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83905-4-RR is Perilipin 1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Perilipin 1 fusion protein Ag26426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name perilipin
Synonyms PLIN, PLIN1, PERI, 240994H1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberNM_001145311
Gene Symbol Perilipin 1
Gene ID (NCBI) 5346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

实验方案

Product Specific Protocols
WB protocol for Perilipin 1 antibody 83905-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols