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PLK1 Recombinant antibody

PLK1 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240092B10

Cat No : 83260-5-RR

Print datasheet

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

验证数据展示

  • WB analysis using 83260-5-RR

    WB analysis using 83260-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83260-5-RR (PLK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83260-5-RR

    IF Staining of HeLa using 83260-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLK1 Ready-To-Use IHC Kit. PLK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83260-5-RR targets PLK1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polo-like kinase 1 (Drosophila)
Synonyms PLK, PLK 1, PLK1, Polo like kinase 1, STPK13
Calculated Molecular Weight 68 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberNM_005030
Gene Symbol PLK1
Gene ID (NCBI) 5347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP53350
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

实验方案

Product Specific Protocols
WB protocol for PLK1 antibody 83260-5-RRDownload protocol
IF protocol for PLK1 antibody 83260-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols