PLK1 Recombinant antibody
PLK1 Recombinant Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
240092B10
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells |
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PLK1 Ready-To-Use IHC Kit. PLK1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83260-5-RR targets PLK1 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | polo-like kinase 1 (Drosophila) |
Synonyms | PLK, PLK 1, PLK1, Polo like kinase 1, STPK13 |
Calculated Molecular Weight | 68 kDa |
Observed Molecular Weight | 66 kDa |
GenBank Accession Number | NM_005030 |
Gene Symbol | PLK1 |
Gene ID (NCBI) | 5347 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P53350 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).
实验方案
Product Specific Protocols | |
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WB protocol for PLK1 antibody 83260-5-RR | Download protocol |
IF protocol for PLK1 antibody 83260-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |