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PLK3 Polyclonal antibody

PLK3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30486-1-AP

Print datasheet

Synonyms

PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase

验证数据展示

  • WB analysis using 30486-1-AP

    WB analysis using 30486-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30486-1-AP (PLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 30486-1-AP

    IP experiment of HeLa using 30486-1-AP

    IP result of anti-PLK3 (IP:30486-1-AP, 4ug; Detection:30486-1-AP 1:500) with HeLa cells lysate 1750 ug.

经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells, NCI-H1299 cells, U2OS cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLK3 Ready-To-Use IHC Kit. PLK3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30486-1-AP targets PLK3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen PLK3 fusion protein Ag33186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polo-like kinase 3 (Drosophila)
Synonyms PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC013899
Gene Symbol PLK3
Gene ID (NCBI) 1263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

实验方案

Product Specific Protocols
WB protocol for PLK3 antibody 30486-1-APDownload protocol
IP protocol for PLK3 antibody 30486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols