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PLOD3 Monoclonal antibody

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2G12B8

Cat no : 60058-1-Ig

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Synonyms

Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase



经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, human placenta tissue, PC-3 cells, K-562 cells, LNCaP cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

60058-1-Ig targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PLOD3 fusion protein Ag1480 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC011674
Gene Symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2165779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60568
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

实验方案

Product Specific Protocols
WB protocol for PLOD3 antibody 60058-1-IgDownload protocol
IHC protocol for PLOD3 antibody 60058-1-IgDownload protocol
IF protocol for PLOD3 antibody 60058-1-IgDownload protocol
IP protocol for PLOD3 antibody 60058-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.

Authors - Stephen A Watt
mouseWB

Matrix Biol Plus

Use of osteoblast-derived matrix to assess the influence of collagen modifications on cancer cells.

Authors - Neus Bota-Rabassedas
mouseWB

JCI Insight

Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.

Authors - Xiaochao Tan