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验证数据展示

  • WB analysis using 21482-1-AP

    WB analysis using 21482-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21482-1-AP (PLP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 21482-1-AP

    IHC staining of human colon using 21482-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21482-1-AP (PLP2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PLP2 antibody included
  • IF Staining of HepG2 using 21482-1-AP

    IF Staining of HepG2 using 21482-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21482-1-AP (PLP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PLP2 Polyclonal antibody

PLP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21482-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Proteolipid protein 2, Differentiation-dependent protein A4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSMMC-7721 cells, HEK-293 cells, COLO 320 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PLP2 Ready-To-Use IHC Kit. PLP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21482-1-AP targets PLP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 PLP2 fusion protein Ag15740 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteolipid protein 2 (colonic epithelium-enriched)
别名 Proteolipid protein 2, Differentiation-dependent protein A4
计算分子量 152 aa, 17 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC109066
基因名称 PLP2
Gene ID (NCBI) 5355
RRIDAB_10858923
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLP2 antibody 21482-1-APDownload protocol
IHC protocol for PLP2 antibody 21482-1-APDownload protocol
IF protocol for PLP2 antibody 21482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Physiol

The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.

Authors - Xiaojian Li
  • KD Validated
View Article
humanIHC

Biomed Res Int

PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma.

Authors - Hua Bai
View Article