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PLRG1 Polyclonal antibody

PLRG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11914-1-AP

Print datasheet

Synonyms

Pleiotropic regulator 1, PLRG1, PRL1

验证数据展示

  • IHC staining of human brain using 11914-1-AP

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 11914-1-AP

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 11914-1-AP

    WB analysis of K-562 using 11914-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11914-1-AP

    IP experiment of HEK-293 using 11914-1-AP

    IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug.

  • WB analysis of HEK-293 using 11914-1-AP

    WB analysis of HEK-293 using 11914-1-AP

    WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, BxPC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11914-1-AP targets PLRG1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PLRG1 fusion protein Ag2527 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
Synonyms Pleiotropic regulator 1, PLRG1, PRL1
Calculated Molecular Weight 514 aa, 57 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC020786
Gene Symbol PLRG1
Gene ID (NCBI) 5356
RRIDAB_2165608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43660
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

实验方案

Product Specific Protocols
WB protocol for PLRG1 antibody 11914-1-APDownload protocol
IHC protocol for PLRG1 antibody 11914-1-APDownload protocol
IP protocol for PLRG1 antibody 11914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

Cell Death Differ

USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

Authors - Shuyan Liu
View Article