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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13143-1-AP (same clone as 13143-1-PBS)

    WB analysis using 13143-1-AP (same clone as 13143-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13143-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

    IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

    IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

  • IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

    IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

  • IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

    IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 13143-1-AP (same clone as 13143-1-PBS)

    IF Staining of A549 using 13143-1-AP (same clone as 13143-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLS1 antibody (13143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

PLS1 Polyclonal antibody, PBS Only

PLS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13143-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13143-1-PBS targets PLS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PLS1 fusion protein Ag3532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plastin 1 (I isoform)
别名
计算分子量 629 aa, 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC031083
基因名称 PLS1
Gene ID (NCBI) 5357
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14651
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLS1 also known as Fimbrin, Plastin 1, is an actin-binding protein in the absence of calcium. In the inner ear, PLS1 is required for stereocilia formation. PLS1 Mediates liquid packing of actin filaments that is necessary for stereocilia to grow to their proper dimensions. PLS1 is specifically expressed at high levels in the small intestine, colon, and kidney; relatively expressed at lower levels in the lung and stomach(PMID: 8139549). PLS1 is required for normal hearing in humans and mice. Mutations in PLS1, encoding fimbrin, cause autosomal dominant nonsyndromic hearing loss(NSHL)(PMID: 31397523).