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验证数据展示

  • WB analysis of mouse small intestine using 55212-1-AP

    WB analysis of mouse small intestine using 55212-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55212-1-AP

    IHC staining of human colon cancer using 55212-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PLS1 Polyclonal antibody

PLS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55212-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse small intestine tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55212-1-AP targets PLS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plastin 1 (I isoform)
别名 Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号NM_002670
基因名称 PLS1
Gene ID (NCBI) 5357
RRIDAB_10859080
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.

实验方案

Product Specific Protocols
WB protocol for PLS1 antibody 55212-1-APDownload protocol
IHC protocol for PLS1 antibody 55212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Sci

Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway.

Authors - Tongtong Zhang
  • KD Validated
View Article