验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse lung tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, HEK-293 cells, HepG2 cells, human placenta tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse spleen tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PLSCR1 fusion protein Ag2172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipid scramblase 1 |
| 别名 | phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1 |
| 计算分子量 | 318 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC021100 |
| 基因名称 | PLSCR1 |
| Gene ID (NCBI) | 5359 |
| RRID | AB_2165659 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15162 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| IHC protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| IF protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| IP protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms | ||
PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication. | ||
J Neurosci Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses
| ||
J Proteome Res An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.
| ||
Cancer Sci Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.
| ||
J Leukoc Biol Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation. |
