PLSCR3 Polyclonal antibody

PLSCR3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28028-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, rat and More (1)

应用

WB, IHC, ELISA

phospholipid scramblase 3, PL scramblase 3, PLSCR3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, A431 cells, mouse liver tissue, mouse lung tissue, rat kidney tissue, rat liver tissue, rat lung tissue, BxPC-3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28028-1-AP targets PLSCR3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, rat
文献引用反应性mouse
免疫原 PLSCR3 fusion protein Ag27655 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipid scramblase 3
别名 phospholipid scramblase 3, PL scramblase 3, PLSCR3
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC011735
基因名称 PLSCR3
Gene ID (NCBI) 57048
RRIDAB_2881040
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRY6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLSCR3, also named as PLS3, belongs to the phospholipid scramblase family. It may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. PLSCR3 may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It is a target of protein kinase C-δ (PKC-δ), resides in mitochondria and plays pivotal roles in regulating apoptotic response. Phosphorylation of PLSCR3 by PKC-δ is involved in the hyperthermia-induced apoptotic signal transduction pathway in Tca8113 cells, and that Hsp27 blocks this pathway to suppress hyperthermia-induced apoptosis. (PMID:20569108)

实验方案

Product Specific Protocols
WB protocol for PLSCR3 antibody 28028-1-APDownload protocol
IHC protocol for PLSCR3 antibody 28028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Cardiolipin externalization mediates prion protein (PrP) peptide 106-126-associated mitophagy and mitochondrial dysfunction

Authors - Dongming Yang
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