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验证数据展示

  • WB analysis using 84592-1-RR

    WB analysis using 84592-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84592-1-RR

    IHC staining of mouse brain using 84592-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84592-1-RR

    IHC staining of mouse brain using 84592-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84592-1-RR

    Affinity and Kinetic Characterization of 84592-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

PLTP Recombinant antibody

PLTP Recombinant Antibody for WB, IHC, ELISA
Cat No. 84592-1-RR

产品说明书

CloneNo. 241583F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Phospholipid transfer protein, Lipid transfer protein II, 241583F3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84592-1-RR targets PLTP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PLTP fusion protein Ag8171 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phospholipid transfer protein
别名 Phospholipid transfer protein, Lipid transfer protein II, 241583F3
计算分子量 493 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC005045
基因名称 PLTP
Gene ID (NCBI) 5360
RRIDAB_3672088
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP55058
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).

实验方案

Product Specific Protocols
WB protocol for PLTP antibody 84592-1-RRDownload protocol
IHC protocol for PLTP antibody 84592-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols