PLXNB1 Polyclonal antibody

PLXNB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23795-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (1)

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLXNB1 Ready-To-Use IHC Kit. PLXNB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23795-1-AP targets PLXNB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 PLXNB1 fusion protein Ag20719 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plexin B1
别名 KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP
计算分子量 2135 aa, 232 kDa
观测分子量 210 kDa
GenBank蛋白编号BC146793
基因名称 PLXNB1
Gene ID (NCBI) 5364
RRIDAB_2879326
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43157
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

实验方案

Product Specific Protocols
WB protocol for PLXNB1 antibody 23795-1-APDownload protocol
IHC protocol for PLXNB1 antibody 23795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Cancer Immunol Res

Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function.

Authors - Paul E Clavijo
humanWB

J Transl Med

SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling.

Authors - Lu Liu
ratWB

Ann Transl Med

Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.

Authors - Kun Wang
mouseIF

J Neuroendocrinol

PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism.

Authors - Bradley A Welch
humanIHC

Ann Transl Med

Methylation-based epigenetic studies and gene integration analysis of preeclampsia

Authors - Lei Jiang
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