验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse liver tissue, mouse ovary tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10602-1-AP targets Plexin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken, goat |
| 免疫原 | Plexin B2 fusion protein Ag0941 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | plexin B2 |
| 别名 | PLXNB2, Plexin-B2, PLEXB2, Nbla00445, MM1 |
| 计算分子量 | 205 kDa |
| 观测分子量 | 80 kDa, 240 kDa |
| GenBank蛋白编号 | BC004542 |
| 基因名称 | PLXNB2 |
| Gene ID (NCBI) | 23654 |
| RRID | AB_2166424 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15031 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IHC protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IF protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IP protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Neuroblastoma plasticity during metastatic progression stems from the dynamics of an early sympathetic transcriptomic trajectory | ||
Nat Commun Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.
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EMBO J Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.
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Hum Reprod Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage | ||
J Periodontal Res Plexin-B2 Mediates Orthodontic Tension-Induced Osteogenesis via the RhoA/F-Actin/YAP Pathway | ||
bioRxiv Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis
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