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Plexin B2 Polyclonal antibody
Plexin B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse liver tissue, mouse ovary tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10602-1-AP targets Plexin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Immunogen | Plexin B2 fusion protein Ag0941 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | plexin B2 |
| Synonyms | PLXNB2, Plexin-B2, PLEXB2, Nbla00445, MM1 |
| Calculated Molecular Weight | 205 kDa |
| Observed Molecular Weight | 80 kDa, 240 kDa |
| GenBank Accession Number | BC004542 |
| Gene Symbol | PLXNB2 |
| Gene ID (NCBI) | 23654 |
| RRID | AB_2166424 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IHC protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IF protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| IP protocol for Plexin B2 antibody 10602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.
| ||
EMBO J Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.
| ||
bioRxiv Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis
| ||
Cell Death Discov Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers | ||
Antiviral Res Harnessing host enhancers of SARS-CoV-2 entry as novel targets for antiviral therapy | ||
Cancer Genomics Proteomics Terrein Exhibits Anti-tumor Activity by Suppressing Angiogenin Expression in Malignant Melanoma Cells |
