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验证数据展示

  • WB analysis of HeLa using 17705-1-AP

    WB analysis of HeLa using 17705-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17705-1-AP (PM20D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17705-1-AP

    IHC staining of human placenta using 17705-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17705-1-AP (PM20D2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 17705-1-AP

    IHC staining of human placenta using 17705-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17705-1-AP (PM20D2 antibody) at dilution of 1:50 (under 40x lens).

PM20D2 Polyclonal antibody

PM20D2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17705-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACY1L2, Aminoacylase 1 like protein 2, PM20D2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human kidney tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17705-1-AP targets PM20D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PM20D2 fusion protein Ag11991 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase M20 domain containing 2
别名 ACY1L2, Aminoacylase 1 like protein 2, PM20D2
计算分子量 436 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC035036
基因名称 PM20D2
Gene ID (NCBI) 135293
RRIDAB_2299854
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PM20D2 antibody 17705-1-APDownload protocol
IHC protocol for PM20D2 antibody 17705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols