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PMCA2 Polyclonal antibody

PMCA2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28423-1-AP

Print datasheet

Synonyms

ATP2B2, PMCA2, PMCA2a, PMCA2i

验证数据展示

  • WB analysis using 28423-1-AP

    WB analysis using 28423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28423-1-AP (PMCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28423-1-AP

    IHC staining of mouse brain using 28423-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 28423-1-AP

    IHC staining of rat brain using 28423-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 28423-1-AP

    IF Staining of rat brain using 28423-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PMCA2 antibody (28423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28423-1-AP targets PMCA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PMCA2 fusion protein Ag28994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 2
Synonyms ATP2B2, PMCA2, PMCA2a, PMCA2i
Calculated Molecular Weight 137 kDa
Observed Molecular Weight 137 kDa
GenBank Accession NumberNM_001001331
Gene Symbol PMCA2
Gene ID (NCBI) 491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

实验方案

Product Specific Protocols
WB protocol for PMCA2 antibody 28423-1-APDownload protocol
IHC protocol for PMCA2 antibody 28423-1-APDownload protocol
IF protocol for PMCA2 antibody 28423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols